› 8iug

Electron Microscopy
2.86Å resolution

Cryo-EM structure of the RC-LH core complex from roseiflexus castenholzii

Released:
DOI: 10.2210/pdb8iug/pdb
PDB entry 8iug coloured by chain, front view.
PDB entry 8iug coloured by chain, side view.
PDB entry 8iug coloured by chain, top view.
Source organism: Roseiflexus castenholzii
Primary publication:
New insights on the photocomplex of Roseiflexus castenholzii revealed from comparisons of native and carotenoid-depleted complexes.
Qi CH, Wang GL, Wang FF, Xin Y, Zou MJ, Madigan MT, Wang-Otomo ZY, Ma F, Yu LJ
OpenAccess logo J Biol Chem 299 105057 (2023)
PMID: 37468106
Related structures: EMD-35721

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 37-mer (preferred)
PDBe Complex ID:
PDB-CPX-501220 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Antenna complex alpha/beta subunit domain-containing protein Chains: 0, 2, 4, 6, 8, B, E, G, I, K, O, Q, S, U, W
Molecule details ›
Chains: 0, 2, 4, 6, 8, B, E, G, I, K, O, Q, S, U, W
Length: 55 amino acids
Theoretical weight: 6.43 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: A7NQE9 (Residues: 1-55; Coverage: 100%)
Gene name: Rcas_3756
Sequence domains: Antenna complex alpha/beta subunit
Alpha subunit of light-harvesting 1 Chains: 1, 3, 5, 7, 9, A, D, F, H, J, N, P, R, T, V
Molecule details ›
Chains: 1, 3, 5, 7, 9, A, D, F, H, J, N, P, R, T, V
Length: 42 amino acids
Theoretical weight: 4.72 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: Q83XD1 (Residues: 1-42; Coverage: 100%)
Gene name: pufA
Cytochrome subunit of photosynthetic reaction center Chain: C
Molecule details ›
Chain: C
Length: 320 amino acids
Theoretical weight: 34.92 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: Q83XC9 (Residues: 1-320; Coverage: 100%)
Gene name: pufC
Sequence domains: Photosynthetic reaction centre cytochrome C subunit
Reaction center protein L chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 641 amino acids
Theoretical weight: 71.71 KDa
Source organism: Roseiflexus castenholzii
UniProt:
  • Canonical: Q83XD0 (Residues: 1-641; Coverage: 100%)
Gene name: pufLM
Sequence domains: Photosynthetic reaction centre protein
TMx polypeptide Chain: X
Molecule details ›
Chain: X
Length: 30 amino acids
Theoretical weight: 3.38 KDa
Source organism: Roseiflexus castenholzii
reaction center small polypeptide Chain: Y
Molecule details ›
Chain: Y
Length: 39 amino acids
Theoretical weight: 4.49 KDa
Source organism: Roseiflexus castenholzii
reaction center unknown polypeptide Chain: Z
Molecule details ›
Chain: Z
Length: 10 amino acids
Theoretical weight: 741 Da
Source organism: Roseiflexus castenholzii
reaction center small polypeptide Chain: h
Molecule details ›
Chain: h
Length: 63 amino acids
Theoretical weight: 6.93 KDa
Source organism: Roseiflexus castenholzii

Ligands and Environments

14 bound ligands:
Ligand BCL 48 x BCL
Ligand U42 14 x U42
Ligand U4Z 16 x U4Z
Ligand LMT 3 x LMT
Ligand BGL 13 x BGL
Ligand HEC 4 x HEC
Ligand CA 1 x CA
Ligand PGV 9 x PGV
Ligand UNL 17 x UNL
Ligand BPH 3 x BPH
Ligand MQE 2 x MQE
Ligand PEF 1 x PEF
Ligand MN 1 x MN
Ligand LHG 1 x LHG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.86Å
Relevant EMDB volumes: EMD-35721

Quick links

Citations

1 review citation

What We Are Learning from the Diverse Structures of the Homodimeric Type I Reaction Center-Photosystems of Anoxygenic Phototropic Bacteria.
Niederman RA. (2024)
1 other article

1 mention without citation

New insights on the photocomplex of Roseiflexus castenholzii revealed from comparisons of native and carotenoid-depleted complexes.
Qi et al. (2023)