8jit

Electron Microscopy
2.91Å resolution

Cryo-EM structure of the GLP-1R/GCGR dual agonist MEDI0382-bound human GCGR-Gs complex

Released:
Primary publication:
Structural analysis of the dual agonism at GLP-1R and GCGR.
OpenAccess logo Proc Natl Acad Sci U S A 120 e2303696120 (2023)
PMID: 37549266
Related structures: EMD-36327

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-546017 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
MEDI0382 Chain: P
Molecule details ›
Chain: P
Length: 29 amino acids
Theoretical weight: 3.31 KDa
Source organism: synthetic construct
Expression system: Not provided
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Nanobody 35 Chain: N
Glucagon receptor Chain: R

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.91Å
Relevant EMDB volumes: EMD-36327
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Escherichia coli