› 8jm5

X-ray diffraction
1.9Å resolution

Endo-deglycosylated hydroxynitrile lyase isozyme 5 mutant L331A/S333V/P340L from Prunus communis

Released:
DOI: 10.2210/pdb8jm5/pdb
PDB entry 8jm5 coloured by chain, front view.
PDB entry 8jm5 coloured by chain, side view.
PDB entry 8jm5 coloured by chain, top view.
Source organism: Prunus dulcis
Entry authors: Zheng Y-C, Li F-L, Yu H-L, Xu J-H

Function and Biology Details

Reaction catalysed: 
(R)-mandelonitrile = cyanide + benzaldehyde
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-247001 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(R)-mandelonitrile lyase Chain: B
Molecule details ›
Chain: B
Length: 540 amino acids
Theoretical weight: 59 KDa
Source organism: Prunus dulcis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q7XJE8 (Residues: 26-559; Coverage: 100%)
Gene name: hnl5
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
Ligand PEG 1 x PEG
Ligand NAG 6 x NAG
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 49.492Å b: 90.95Å c: 130.435Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.19
Expression system: Komagataella pastoris

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