› 8p31

Electron Microscopy
3.24Å resolution

Structure of human SIT1:ACE2 complex (closed PD conformation) bound to L-pipecolate

Released:
DOI: 10.2210/pdb8p31/pdb
PDB entry 8p31 coloured by chain, front view.
PDB entry 8p31 coloured by chain, side view.
PDB entry 8p31 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of the SIT1 proline transporter in complex with the COVID-19 receptor ACE2.
Li HZ, Pike ACW, Lotsaris I, Chi G, Hansen JS, Lee SC, Rödström KEJ, Bushell SR, Speedman D, Evans A, Wang D, He D, Shrestha L, Nasrallah C, Burgess-Brown NA, Vandenberg RJ, Dafforn TR, Carpenter EP, Sauer DB
Nat Commun 15 5503 (2024)
PMID: 38951531
Related structures: EMD-17382

Function and Biology Details

Reaction catalysed: 
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230395 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Angiotensin-converting enzyme 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 812 amino acids
Theoretical weight: 93.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BYF1 (Residues: 1-17, 18-805; Coverage: 100%)
Gene names: ACE2, UNQ868/PRO1885
Sequence domains:
Sodium- and chloride-dependent transporter XTRP3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 641 amino acids
Theoretical weight: 71.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NP91 (Residues: 1-592; Coverage: 100%)
Gene names: SIT1, SLC6A20, XT3, XTRP3
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand ZN 2 x ZN
Ligand NDG 2 x NDG
Ligand NAG 12 x NAG
Ligand YCP 2 x YCP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.24Å
Relevant EMDB volumes: EMD-17382
Expression system: Homo sapiens

Quick links

Citations

2 citation in other articles

In silico structural studies on the vesicular neutral amino acid transporter NTT4 (SLC6A17).
Kukułowicz et al. (2024)
1 more

1 mention without citation

Structure and function of the SIT1 proline transporter in complex with the COVID-19 receptor ACE2.
Li et al. (2024)