› 8pvb

Electron Microscopy
3.6Å resolution

Structure of GABAAR determined by cryoEM at 100 keV

Released:
DOI: 10.2210/pdb8pvb/pdb
PDB entry 8pvb coloured by chain, front view.
PDB entry 8pvb coloured by chain, side view.
PDB entry 8pvb coloured by chain, top view.
Source organisms:
Primary publication:
Structure determination by cryoEM at 100 keV.
McMullan G, Naydenova K, Mihaylov D, Yamashita K, Peet MJ, Wilson H, Dickerson JL, Chen S, Cannone G, Lee Y, Hutchings KA, Gittins O, Sobhy MA, Wells T, El-Gomati MM, Dalby J, Meffert M, Schulze-Briese C, Henderson R, Russo CJ
OpenAccess logo Proc Natl Acad Sci U S A 120 e2312905120 (2023)
PMID: 38011573
Related structures: EMD-17960

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-276187 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Gamma-aminobutyric acid receptor subunit beta-3 Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 45.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P28472 (Residues: 26-332; Coverage: 69%)
  • Best match: P28472-4 (Residues: 1-247)
Gene name: GABRB3
Sequence domains:
Megabody Mb25 Chain: O
Molecule details ›
Chain: O
Length: 522 amino acids
Theoretical weight: 56.3 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
5 bound ligands:
Ligand R16 1 x R16
Ligand D10 4 x D10
Ligand HSM 1 x HSM
Ligand CL 2 x CL
Ligand V8D 1 x V8D
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-17960
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

1 review citation

Cryo-electron microscopy-based drug design.
Cebi et al. (2024)
4 other articles

1 mention without citation

VHH Nanobody Versatility against Pentameric Ligand-Gated Ion Channels.
Nemecz et al. (2024)