› 8pwh

Electron Microscopy
3.17Å resolution

Atomic structure and conformational variability of the HER2-Trastuzumab-Pertuzumab complex

Released:
DOI: 10.2210/pdb8pwh/pdb
PDB entry 8pwh coloured by chain, front view.
PDB entry 8pwh coloured by chain, side view.
PDB entry 8pwh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and conformational variability of the HER2-trastuzumab-pertuzumab complex.
Ruedas R, Vuillemot R, Tubiana T, Winter JM, Pieri L, Arteni AA, Samson C, Jonic S, Mathieu M, Bressanelli S
J Struct Biol 216 108095 (2024)
PMID: 38723875
Related structures: EMD-17993

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240206 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Trastuzumab Fab light chain Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.47 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Trastuzumab Fab heavy chain Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 23.43 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Pertuzumab Fab light chain Chain: C
Molecule details ›
Chain: C
Length: 214 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Pertuzumab Fab heavy chain Chain: D
Molecule details ›
Chain: D
Length: 222 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Receptor tyrosine-protein kinase erbB-2 Chain: E
Molecule details ›
Chain: E
Length: 624 amino acids
Theoretical weight: 68.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P04626 (Residues: 23-646; Coverage: 51%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.17Å
Relevant EMDB volumes: EMD-17993
Expression systems:
  • Cricetulus griseus
  • Homo sapiens

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