› 8qhl

X-ray diffraction
1.9Å resolution

Human Angiotensin-1 converting enzyme N-domain in complex with the lactotripeptide VPP

Released:
DOI: 10.2210/pdb8qhl/pdb
PDB entry 8qhl coloured by chain, front view.
PDB entry 8qhl coloured by chain, side view.
PDB entry 8qhl coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the inhibitory mechanism of angiotensin-I-converting enzyme by the lactotripeptides IPP and VPP.
Gregory KS, Cozier GE, Schwager SLU, Sturrock ED, Acharya KR
FEBS Lett (2023)
PMID: 37904282

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-537004 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Angiotensin-converting enzyme, soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 628 amino acids
Theoretical weight: 72.49 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12821 (Residues: 30-657; Coverage: 49%)
Gene names: ACE, DCP, DCP1
Sequence domains: Angiotensin-converting enzyme
VAL-PRO-PRO Chains: C, D
Molecule details ›
Chains: C, D
Length: 3 amino acids
Theoretical weight: 311 Da
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
9 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand PEG 6 x PEG
Ligand EDO 2 x EDO
Ligand PG4 2 x PG4
Ligand PGE 1 x PGE
Ligand BCN 1 x BCN
Ligand MG 1 x MG
Ligand 1PE 2 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 73.337Å b: 78.222Å c: 83.011Å
α: 88.75° β: 64.703° γ: 75.248°
R-values:
R R work R free
0.191 0.188 0.233
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

1 citations in other articles

Ile-Pro-Pro attenuates sympathetic activity and hypertension.
Chen et al. (2024)

2 mentions without citation

Advances in the structural basis for angiotensin-1 converting enzyme (ACE) inhibitors.
Acharya et al. (2024)
1 more