› 8qst

Electron Microscopy
2.89Å resolution

Cryo-EM structure of the glucose-specific PTS transporter IICB from E. coli in the inward- and outward-facing conformation

Released:
DOI: 10.2210/pdb8qst/pdb
PDB entry 8qst coloured by chain, front view.
PDB entry 8qst coloured by chain, side view.
PDB entry 8qst coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Roth P, Fotiadis D, Jeckelmann J-M
Related structures: EMD-18641

Function and Biology Details

Reaction catalysed: 
[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-550189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system glucose-specific EIICB component Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 53.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69786 (Residues: 1-477; Coverage: 100%)
Gene names: JW1087, b1101, glcA, ptsG, umg
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand BGC 2 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.89Å
Relevant EMDB volumes: EMD-18641
Expression system: Escherichia coli

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