› 8saw

Electron Microscopy
3.3Å resolution

CryoEM structure of DH270.UCA.G57R-CH848.10.17DT

Released:
DOI: 10.2210/pdb8saw/pdb
PDB entry 8saw coloured by chain, front view.
PDB entry 8saw coloured by chain, side view.
PDB entry 8saw coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for breadth development in the HIV-1 V3-glycan targeting DH270 antibody clonal lineage.
Henderson R, Zhou Y, Stalls V, Wiehe K, Saunders KO, Wagh K, Anasti K, Barr M, Parks R, Alam SM, Korber B, Haynes BF, Bartesaghi A, Acharya P
OpenAccess logo Nat Commun 14 2782 (2023)
PMID: 37188681
Related structures: EMD-40282

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-496175 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Envelope glycoprotein gp160 Chains: A, E, K
Molecule details ›
Chains: A, E, K
Length: 471 amino acids
Theoretical weight: 52.85 KDa
Source organism: HIV-1 06TG.HT008
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A1W6IPB2 (Residues: 30-495; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Envelope glycoprotein gp160 Chains: B, F, L
Molecule details ›
Chains: B, F, L
Length: 132 amino acids
Theoretical weight: 14.73 KDa
Source organism: HIV-1 06TG.HT008
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A6H1VLP0 (Residues: 460-612; Coverage: 20%)
Gene name: env
Sequence domains: Retroviral envelope protein
DH270.UCA. G57R heavy chain Chains: C, H, M
Molecule details ›
Chains: C, H, M
Length: 127 amino acids
Theoretical weight: 14.24 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
DH270.UCA. G57R light chain Chains: D, I, N
Molecule details ›
Chains: D, I, N
Length: 110 amino acids
Theoretical weight: 11.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-40282
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Progress with induction of HIV broadly neutralizing antibodies in the Duke Consortia for HIV/AIDS Vaccine Development.
Haynes et al. (2023)
2 more

1 mention without citation

Kinetic studies and homology modeling of a dual-substrate linalool/nerolidol synthase from Plectranthus amboinicus.
Ashaari et al. (2021)