› 8thm

X-ray diffraction
2.3Å resolution

Beta carbonic anhydrase from the carboxysome of Cyanobium PCC 7001

Released:
DOI: 10.2210/pdb8thm/pdb
PDB entry 8thm coloured by chain, front view.
PDB entry 8thm coloured by chain, side view.
PDB entry 8thm coloured by chain, top view.
Source organism: Cyanobium sp. PCC 7001
Primary publication:
Cyanobacterial α-carboxysome carbonic anhydrase is allosterically regulated by the Rubisco substrate RuBP.
Pulsford SB, Outram MA, Förster B, Rhodes T, Williams SJ, Badger MR, Price GD, Jackson CJ, Long BM
Sci Adv 10 eadk7283 (2024)
PMID: 38728392

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxysome shell carbonic anhydrase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 462 amino acids
Theoretical weight: 51.31 KDa
Source organism: Cyanobium sp. PCC 7001
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5ILN4 (Residues: 94-553; Coverage: 82%)
Gene name: CPCC7001_23
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand ZN 8 x ZN
Ligand GOL 3 x GOL
Ligand SO4 18 x SO4
Ligand EDO 10 x EDO
Ligand RUB 6 x RUB
Ligand CO2 2 x CO2
Ligand BCT 1 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 104.314Å b: 181.848Å c: 190.057Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links