› 8tnt

X-ray diffraction
3.15Å resolution

Crystal structure of Epstein-Barr virus gH/gL/gp42 in complex with antibodies F-2-1 and 769C2

Released:
DOI: 10.2210/pdb8tnt/pdb
Model geometry
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PDB entry 8tnt coloured by chain, front view.
PDB entry 8tnt coloured by chain, side view.
PDB entry 8tnt coloured by chain, top view.
Source organisms:
Primary publication:
Epstein-Barr virus gp42 antibodies reveal sites of vulnerability for receptor binding and fusion to B cells.
Bu W, Kumar A, Board NL, Kim J, Dowdell K, Zhang S, Lei Y, Hostal A, Krogmann T, Wang Y, Pittaluga S, Marcotrigiano J, Cohen JI
Immunity 57 559-573.e6 (2024)
PMID: 38479361

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-242264 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Envelope glycoprotein H Chain: A
Molecule details ›
Chain: A
Length: 656 amino acids
Theoretical weight: 72.69 KDa
Source organism: human gammaherpesvirus 4
Expression system: Homo sapiens
UniProt:
  • Canonical: P03231 (Residues: 19-674; Coverage: 95%)
Gene names: BXLF2, gH
Sequence domains:
Envelope glycoprotein L Chain: B
Molecule details ›
Chain: B
Length: 112 amino acids
Theoretical weight: 12.48 KDa
Source organism: human gammaherpesvirus 4
Expression system: Homo sapiens
UniProt:
  • Canonical: P03212 (Residues: 24-135; Coverage: 97%)
Gene names: BKRF2, gL
Sequence domains: Viral glycoprotein L
Soluble gp42 Chain: C
769C2 light chain Chain: D
769C2 heavy chain Chain: E
F-2-1 light chain Chain: F
F-2-1 heavy chain Chain: G

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 117.317Å b: 85.172Å c: 119.714Å
α: 90° β: 105.09° γ: 90°
R-values:
R R work R free
0.256 0.254 0.287
Expression system: Homo sapiens

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Citations

3 citation in other articles

A gH/gL-encoding replicon vaccine elicits neutralizing antibodies that protect humanized mice against EBV challenge.
Edwards et al. (2024)
2 more

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