› 8txp

X-ray diffraction
2.75Å resolution

Crystal structure of 05.GC.w13.01 Fab in complex with H1 HA from A/California/04/2009(H1N1)

Released:
DOI: 10.2210/pdb8txp/pdb
PDB entry 8txp coloured by chain, front view.
PDB entry 8txp coloured by chain, side view.
PDB entry 8txp coloured by chain, top view.
Source organisms:
Primary publication:
Maturation of germinal center B cells after influenza virus vaccination in humans.
McIntire KM, Meng H, Lin TH, Kim W, Moore NE, Han J, McMahon M, Wang M, Malladi SK, Mohammed BM, Zhou JQ, Schmitz AJ, Hoehn KB, Carreño JM, Yellin T, Suessen T, Middleton WD, Teefey SA, Presti RM, Krammer F, Turner JS, Ward AB, Wilson IA, Kleinstein SH, Ellebedy AH
J Exp Med 221 (2024)
PMID: 38935072

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-503978 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 36.73 KDa
Source organism: Influenza A virus
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: C3W5S1 (Residues: 18-344; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 20.21 KDa
Source organism: Influenza A virus
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: C3W5S1 (Residues: 345-518; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
GC_w13_A, Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 23.56 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
GC_w13_A, Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P321
Unit cell:
a: 128.35Å b: 128.35Å c: 155.756Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.252 0.25 0.292
Expression systems:
  • Spodoptera aff. frugiperda 1 BOLD-2017
  • Cricetulus griseus

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