› 8tyz

X-ray diffraction
2.68Å resolution

Structure of a bacterial E1-E2-Ubl complex (form 2)

Released:
DOI: 10.2210/pdb8tyz/pdb
PDB entry 8tyz coloured by chain, front view.
PDB entry 8tyz coloured by chain, side view.
PDB entry 8tyz coloured by chain, top view.
Source organism: Ensifer aridi
Primary publication:
A eukaryotic-like ubiquitination system in bacterial antiviral defence.
Chambers LR, Ye Q, Cai J, Gong M, Ledvina HE, Zhou H, Whiteley AT, Suhandynata RT, Corbett KD
Nature (2024)
PMID: 39020180

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245825 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
E1(BilD) Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 56.95 KDa
Source organism: Ensifer aridi
Expression system: Escherichia coli
Ubl(BilA) Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 10.58 KDa
Source organism: Ensifer aridi
Expression system: Escherichia coli
E2(BilB) Chain: C
Molecule details ›
Chain: C
Length: 204 amino acids
Theoretical weight: 22.98 KDa
Source organism: Ensifer aridi
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P42212
Unit cell:
a: 199.925Å b: 199.925Å c: 66.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.245
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Tracking of Ubiquitin Signaling through 3.5 Billion Years of Combinatorial Conjugation.
Kaminskaya et al. (2024)