› 8uw8

Electron Microscopy
2.3Å resolution

Site-one protease and SPRING

Released:
DOI: 10.2210/pdb8uw8/pdb
PDB entry 8uw8 coloured by chain, front view.
PDB entry 8uw8 coloured by chain, side view.
PDB entry 8uw8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SPRING licenses S1P-mediated cleavage of SREBP2 by displacing an inhibitory pro-domain.
Hendrix S, Dartigue V, Hall H, Bawaria S, Kingma J, Bajaj B, Zelcer N, Kober DL
OpenAccess logo Nat Commun 15 5732 (2024)
PMID: 38977690
Related structures: EMD-42639

Function and Biology Details

Reaction catalysed: 
Processes precursors containing basic and hydrophobic/aliphatic residues at P4 and P2, respectively, with a relatively relaxed acceptance of amino acids at P1 and P3
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-556526 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Membrane-bound transcription factor site-1 protease Chain: A
Molecule details ›
Chain: A
Length: 1026 amino acids
Theoretical weight: 115.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q14703 (Residues: 1-998; Coverage: 95%)
Gene names: KIAA0091, MBTPS1, S1P, SKI1
Sequence domains:
SREBP regulating gene protein Chain: B
Molecule details ›
Chain: B
Length: 201 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H741 (Residues: 36-205; Coverage: 83%)
Gene names: C12orf49, SPRING, SPRING1
Sequence domains: SREBP regulating gene protein

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.3Å
Relevant EMDB volumes: EMD-42639
Expression system: Homo sapiens

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