8wg8

Electron Microscopy
2.71Å resolution

Cryo-EM structures of peptide free and Gs-coupled GCGR

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-546014 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-3, 27-67, 205-394; Coverage: 57%)
  • Canonical: P63096 (Residues: 4-19, 61-181; Coverage: 39%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 371 amino acids
Theoretical weight: 40.23 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54311 (Residues: 2-340; Coverage: 100%)
Gene name: Gnb1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Nanobody-35 Chain: N
Glucagon receptor Chain: R
PRO-PRO-PRO-PRO-PHE-SER-ASN-LEU-VAL-MET-ASP-ASP-LEU-LYS-ASN-LYS-LYS Chain: E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.71Å
Relevant EMDB volumes: EMD-37505
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli