› 8wl9

X-ray diffraction
1.93Å resolution

X-ray structure of Enterobacter cloacae allose-binding protein in complex with D-ribose

Released:
DOI: 10.2210/pdb8wl9/pdb
PDB entry 8wl9 coloured by chain, front view.
PDB entry 8wl9 coloured by chain, side view.
PDB entry 8wl9 coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
X-ray structures of Enterobacter cloacae allose-binding protein in complexes with monosaccharides demonstrate its unique recognition mechanism for high affinity to allose.
Kamitori S
Biochem Biophys Res Commun 682 187-192 (2023)
PMID: 37820454

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 32.82 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A9W3L9M6 (Residues: 24-311; Coverage: 100%)
Gene name: ECNIH2_02675
Sequence domains: Periplasmic binding protein domain

Ligands and Environments

1 bound ligand:
Ligand RIP 1 x RIP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21212
Unit cell:
a: 66.85Å b: 110.68Å c: 37.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.29
Expression system: Escherichia coli

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