› 8wlr

Electron Microscopy
3.12Å resolution

Cryo-EM structure of SARS-CoV-2 prototype spike protein receptor-binding domain in complex with hippopotamus ACE2

Released:
DOI: 10.2210/pdb8wlr/pdb
PDB entry 8wlr coloured by chain, front view.
PDB entry 8wlr coloured by chain, side view.
PDB entry 8wlr coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of hippopotamus ACE2 binding to SARS-CoV-2.
Yang R, Han P, Han P, Li D, Zhao R, Niu S, Liu K, Li S, Tian W-X, Gao GF
J Virol 98 e0045124 (2024)
PMID: 38591877
Related structures: EMD-37629

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-272812 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Angiotensin-converting enzyme Chain: A
Molecule details ›
Chain: A
Length: 597 amino acids
Theoretical weight: 69.34 KDa
Source organism: Hippopotamus amphibius
Expression system: Escherichia coli
Spike glycoprotein Chain: B
Molecule details ›
Chain: B
Length: 1217 amino acids
Theoretical weight: 135.12 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1217; Coverage: 96%)
Gene names: 2, S
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.12Å
Relevant EMDB volumes: EMD-37629
Expression systems:
  • Escherichia coli
  • Homo sapiens

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