› 8x05

X-ray diffraction
1.13Å resolution

X-Ray crystal structure of glycoside hydrolase family 6 cellobiohydrolase from Phanerochaete chrysosporium PcCel6A C240S/C393S soaked in cellotriose

Released:
DOI: 10.2210/pdb8x05/pdb
PDB entry 8x05 coloured by chain, front view.
PDB entry 8x05 coloured by chain, side view.
PDB entry 8x05 coloured by chain, top view.
Source organism: Phanerodontia chrysosporium
Entry authors: Yamaguchi S, Sunagawa N, Tachioka M, Igarashi K

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-515761 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucanase Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 38.35 KDa
Source organism: Phanerodontia chrysosporium
Expression system: Komagataella pastoris
UniProt:
  • Canonical: H3K419 (Residues: 82-439; Coverage: 82%)
Gene name: cel6A
Sequence domains: Glycosyl hydrolases family 6

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
1 bound ligand:
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 54.15Å b: 67.296Å c: 85.926Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.207
Expression system: Komagataella pastoris

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