› 8a25

X-ray diffraction
1.73Å resolution

Lysophospholipase PlaA from Legionella pneumophila str. Corby - complex with PEG fragment

Released:
DOI: 10.2210/pdb8a25/pdb
PDB entry 8a25 coloured by chain, front view.
PDB entry 8a25 coloured by chain, side view.
PDB entry 8a25 coloured by chain, top view.
Source organism: Legionella pneumophila str. Corby
Primary publication:
Structure-function relationships underpin disulfide loop cleavage-dependent activation of Legionella pneumophila lysophospholipase A PlaA.
Hiller M, Diwo M, Wamp S, Gutsmann T, Lang C, Blankenfeldt W, Flieger A
Mol Microbiol (2023)
PMID: 38130174

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysophospholipase A Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 35.07 KDa
Source organism: Legionella pneumophila str. Corby
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A378KFD4 (Residues: 19-309; Coverage: 100%)
Gene names: NCTC12000_02489, O6C86_14680
Sequence domains: GDSL-like Lipase/Acylhydrolase

Ligands and Environments

4 bound ligands:
Ligand MLA 2 x MLA
Ligand BU3 1 x BU3
Ligand PGE 1 x PGE
Ligand IOD 1 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 82.238Å b: 82.238Å c: 68.447Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.158 0.183
Expression system: Escherichia coli BL21(DE3)

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