› 8a2r

Electron Microscopy
2.17Å resolution

Cryo-EM structure of F-actin in the Mg2+-ADP-BeF3- nucleotide state.

Released:
DOI: 10.2210/pdb8a2r/pdb
PDB entry 8a2r coloured by chain, front view.
PDB entry 8a2r coloured by chain, side view.
PDB entry 8a2r coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structural basis of actin filament assembly and aging.
Oosterheert W, Klink BU, Belyy A, Pospich S, Raunser S
OpenAccess logo Nature 611 374-379 (2022)
PMID: 36289337
Related structures: EMD-15104

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

3 bound ligands:
Ligand ADP 5 x ADP
Ligand MG 5 x MG
Ligand BEF 5 x BEF
1 modified residue:
Ligand HIC 5 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 2.17Å
Relevant EMDB volumes: EMD-15104

Quick links

Citations

3 review citations

Cytoskeleton Rearrangement in Podocytopathies: An Update.
Ma et al. (2024)
2 more

3 mentions without citation

Actin polymerization and depolymerization in developing vertebrates.
Bai et al. (2023)
2 more