› 8a4a

X-ray diffraction
2.52Å resolution

Structure of human Rep15 in complex with bovine Rab3C.

Released:
DOI: 10.2210/pdb8a4a/pdb
PDB entry 8a4a coloured by chain, front view.
PDB entry 8a4a coloured by chain, side view.
PDB entry 8a4a coloured by chain, top view.
Source organisms:
Primary publication:
Rep15 interacts with several Rab GTPases and has a distinct fold for a Rab effector.
Rai A, Singh AK, Bleimling N, Posern G, Vetter IR, Goody RS
OpenAccess logo Nat Commun 13 4262 (2022)
PMID: 35871249

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-536223 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rab15 effector protein Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 26.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6BDI9 (Residues: 1-236; Coverage: 100%)
Gene name: REP15
Sequence domains: Rab15 effector
Ras-related protein Rab-3C Chain: B
Molecule details ›
Chain: B
Length: 229 amino acids
Theoretical weight: 26.12 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10949 (Residues: 10-227; Coverage: 96%)
Gene name: RAB3C
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I222
Unit cell:
a: 58.29Å b: 108.795Å c: 172.027Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.254
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

The genome of the pygmy right whale illuminates the evolution of rorquals.
Wolf et al. (2023)