› 8a5s

X-ray diffraction
1.85Å resolution

Crystal structure of light-activated DNA-binding protein EL222 from Erythrobacter litoralis crystallized in dark, measured illuminated.

Released:
DOI: 10.2210/pdb8a5s/pdb
PDB entry 8a5s coloured by chain, front view.
PDB entry 8a5s coloured by chain, side view.
PDB entry 8a5s coloured by chain, top view.
Source organism: Erythrobacter litoralis HTCC2594
Entry authors: Koval T, Chaudhari A, Fuertes G, Andersson I, Dohnalek J

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-558254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Light-activated DNA-binding protein EL222 Chain: AAA
Molecule details ›
Chain: AAA
Length: 210 amino acids
Theoretical weight: 23.21 KDa
Source organism: Erythrobacter litoralis HTCC2594
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2NB98 (Residues: 17-225; Coverage: 93%)
Gene name: ELI_04755
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand FMN 1 x FMN
Ligand CL 2 x CL
Ligand MG 3 x MG
Ligand MES 1 x MES
1 modified residue:
Ligand OHI 1 x OHI

Experiments and Validation Details

Entry percentile scores
X-ray source: Excillum MetalJet D2 70 kV
Spacegroup: P212121
Unit cell:
a: 43.426Å b: 51.852Å c: 80.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.215
Expression system: Escherichia coli BL21(DE3)

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