› 8a6n

X-ray diffraction
1.63Å resolution

Room temperature rsEGFP2 with a chlorinated chromophore 300 fs after Photoexcitation

Released:
DOI: 10.2210/pdb8a6n/pdb
Model geometry
Fit model/data
PDB entry 8a6n coloured by chain, front view.
PDB entry 8a6n coloured by chain, side view.
PDB entry 8a6n coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism.
Fadini A, Hutchison CDM, Morozov D, Chang J, Maghlaoui K, Perrett S, Luo F, Kho JCX, Romei MG, Morgan RML, Orr CM, Cordon-Preciado V, Fujiwara T, Nuemket N, Tosha T, Tanaka R, Owada S, Tono K, Iwata S, Boxer SG, Groenhof G, Nango E, van Thor JJ
OpenAccess logo J Am Chem Soc (2023)
PMID: 37418747

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.57 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand OHD 1 x OHD

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SACLA BEAMLINE BL3
Spacegroup: P212121
Unit cell:
a: 51.99Å b: 62.91Å c: 72.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.213
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Structural Characterization of Fluorescent Proteins Using Tunable Femtosecond Stimulated Raman Spectroscopy.
Chen et al. (2023)

1 mention without citation

Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism.
Fadini et al. (2023)

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