› 8a7h

X-ray diffraction
3.14Å resolution

Crystal structure of a chimeric LOV-Histidine kinase SB2F1-I66R mutant (light state; asymmetrical variant, trigonal form with long c axis)

Released:
DOI: 10.2210/pdb8a7h/pdb
PDB entry 8a7h coloured by chain, front view.
PDB entry 8a7h coloured by chain, side view.
PDB entry 8a7h coloured by chain, top view.
Source organisms:
Entry authors: Arinkin V, Granzin J, Batra-Safferling R

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237026 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor protein FixL; Sensory box protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 42.94 KDa
Source organisms: Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q88JB0 (Residues: 4-123; Coverage: 80%)
  • Canonical: P23222 (Residues: 258-505; Coverage: 49%)
Gene names: PP_2739, bll2760, fixL
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand JGC 2 x JGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 139.42Å b: 139.42Å c: 89.881Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.257 0.253 0.306
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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