› 8a8f

X-ray diffraction
1.85Å resolution

Crystal structure of Glc7 phosphatase in complex with the regulatory region of Ref2

Released:
DOI: 10.2210/pdb8a8f/pdb
PDB entry 8a8f coloured by chain, front view.
PDB entry 8a8f coloured by chain, side view.
PDB entry 8a8f coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A direct interaction between CPF and RNA Pol II links RNA 3' end processing to transcription.
Carminati M, Rodríguez-Molina JB, Manav MC, Bellini D, Passmore LA
OpenAccess logo Mol Cell 83 4461-4478.e13 (2023)
PMID: 38029752

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542803 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase PP1-2 Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 36.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P32598 (Residues: 1-312; Coverage: 100%)
Gene names: CID1, DIS2, GLC7, YER133W
Sequence domains:
RNA end formation protein 2 Chain: B
Molecule details ›
Chain: B
Length: 80 amino acids
Theoretical weight: 8.5 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P42073 (Residues: 348-406; Coverage: 11%)
Gene names: REF2, YD9346.06, YDR195W

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3121
Unit cell:
a: 90.23Å b: 90.23Å c: 101.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.22
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Co-transcriptional gene regulation in eukaryotes and prokaryotes.
Shine et al. (2024)
1 other article