› 8aab

X-ray diffraction
1.6Å resolution

S148F mutant of blue-to-red fluorescent timer mRubyFT

Released:
DOI: 10.2210/pdb8aab/pdb
PDB entry 8aab coloured by chain, front view.
PDB entry 8aab coloured by chain, side view.
PDB entry 8aab coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
mRubyFT/S147I, a mutant of blue-to-red fluorescent timer
Boyko KM, Nikolaeva AY, Dorovatovskii PV, Vlaskina AV, Subach OM, Subach FV
Crystallography Reports (2022)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein eqFP611 Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.1 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ISF8 (Residues: 4-221; Coverage: 94%)
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: KURCHATOV SNC BEAMLINE K4.4
Spacegroup: P212121
Unit cell:
a: 31.524Å b: 66.465Å c: 96.488Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.22
Expression system: Escherichia coli

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