› 8act

Electron Microscopy
3.6Å resolution

structure of the human beta-cardiac myosin folded-back off state

Released:
DOI: 10.2210/pdb8act/pdb
PDB entry 8act coloured by chain, front view.
PDB entry 8act coloured by chain, side view.
PDB entry 8act coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the folded-back state of human β-cardiac myosin.
Grinzato A, Auguin D, Kikuti C, Nandwani N, Moussaoui D, Pathak D, Kandiah E, Ruppel KM, Spudich JA, Houdusse A, Robert-Paganin J
OpenAccess logo Nat Commun 14 3166 (2023)
PMID: 37258552
Related structures: EMD-15353

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140206 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 904 amino acids
Theoretical weight: 103.81 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P12883 (Residues: 3-906; Coverage: 47%)
Gene names: MYH7, MYHCB
Sequence domains:
Myosin light chain 3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 157 amino acids
Theoretical weight: 17.9 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08590 (Residues: 39-195; Coverage: 81%)
Gene name: MYL3
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform Chains: E, F
Molecule details ›
Chains: E, F
Length: 144 amino acids
Theoretical weight: 16.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P10916 (Residues: 20-163; Coverage: 87%)
Gene names: MLC2, MYL2
Sequence domains: EF-hand domain pair

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand PO4 2 x PO4
Ligand MG 2 x MG
1 modified residue:
Ligand M3L 4 x M3L

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-15353
Expression system: Homo sapiens

Quick links

Citations

3 citation in other articles

Cryo-EM structure of the human cardiac myosin filament.
Dutta et al. (2023)
2 more

2 mentions without citation

Dilated cardiomyopathy mutation in beta-cardiac myosin enhances actin activation of the power stroke and phosphate release.
Bodt et al. (2023)
1 more