› 8ad4

X-ray diffraction
1.5Å resolution

X-ray structure of NqrF(129-408)of Vibrio cholerae in complex with NADH

Released:
DOI: 10.2210/pdb8ad4/pdb
Model geometry
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PDB entry 8ad4 coloured by chain, front view.
PDB entry 8ad4 coloured by chain, side view.
PDB entry 8ad4 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Conformational coupling of redox-driven Na+-translocation in Vibrio cholerae NADH:quinone oxidoreductase.
Hau JL, Kaltwasser S, Muras V, Casutt MS, Vohl G, Claußen B, Steffen W, Leitner A, Bill E, Cutsail GE, DeBeer S, Vonck J, Steuber J, Fritz G
OpenAccess logo Nat Struct Mol Biol (2023)
PMID: 37710014

Function and Biology Details

Reaction catalysed: 
NADH + ubiquinone + n Na(+)(Side 1) = NAD(+) + ubiquinol + n Na(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)-translocating NADH-quinone reductase subunit F Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 32.28 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A5F5Y4 (Residues: 130-408; Coverage: 68%)
Gene names: VC0395_A1879, VC395_2406, nqrF
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand FAD 2 x FAD
Ligand NAI 5 x NAI
Ligand NA 4 x NA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 72.2Å b: 89.38Å c: 95.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.205
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 citations in other articles

Functionality of the Na+-translocating NADH:quinone oxidoreductase and quinol:fumarate reductase from Prevotella bryantii inferred from homology modeling.
Hau et al. (2023)

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