› 8adj

X-ray diffraction
2.51Å resolution

Poly(ADP-ribose) glycohydrolase (PARG) from Drosophila melanogaster in complex with PARG inhibitor PDD00017272

Released:
DOI: 10.2210/pdb8adj/pdb
PDB entry 8adj coloured by chain, front view.
PDB entry 8adj coloured by chain, side view.
PDB entry 8adj coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Serine ADP-ribosylation in Drosophila provides insights into the evolution of reversible ADP-ribosylation signalling.
Fontana P, Buch-Larsen SC, Suyari O, Smith R, Suskiewicz MJ, Schützenhofer K, Ariza A, Rack JGM, Nielsen ML, Ahel I
OpenAccess logo Nat Commun 14 3200 (2023)
PMID: 37268618

Function and Biology Details

Reaction catalysed: 
Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(ADP-ribose) glycohydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 578 amino acids
Theoretical weight: 65.99 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O46043 (Residues: 1-558; Coverage: 77%)
Gene names: CG2864, Parg
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand LQX 3 x LQX
Ligand GOL 5 x GOL
Ligand SO4 6 x SO4
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 93.392Å b: 115.919Å c: 123.267Å
α: 90° β: 112.205° γ: 90°
R-values:
R R work R free
0.185 0.183 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities.
Đukić et al. (2023)

1 mention without citation

Serine ADP-ribosylation in Drosophila provides insights into the evolution of reversible ADP-ribosylation signalling.
Fontana et al. (2023)