› 8aey

Electron Microscopy
3.05Å resolution

3 A CRYO-EM STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS FERRITIN FROM TIMEPIX3 detector

Released:
DOI: 10.2210/pdb8aey/pdb
PDB entry 8aey coloured by chain, front view.
PDB entry 8aey coloured by chain, side view.
PDB entry 8aey coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Integration of an Event-driven Timepix3 Hybrid Pixel Detector into a Cryo-EM Workflow
van Schayck JP, Zhang Y, Knoops K, Peters PJ, Ravelli RBG
Microsc Microanal (2023)
Related structures: EMD-15389

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterioferritin BfrB Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, V, W, X, Y
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, V, W, X, Y
Length: 181 amino acids
Theoretical weight: 20.46 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNE5 (Residues: 1-181; Coverage: 100%)
Gene names: Rv3841, bfrB, ftn
Sequence domains: Ferritin-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.05Å
Relevant EMDB volumes: EMD-15389
Expression system: Escherichia coli BL21(DE3)

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