› 8ahm

X-ray diffraction
2.42Å resolution

Crystal structure of tubulin in complex with C(13)/C(13')-Bis-Desmethyl-Disorazole Z

Released:
DOI: 10.2210/pdb8ahm/pdb
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PDB entry 8ahm coloured by chain, front view.
PDB entry 8ahm coloured by chain, side view.
PDB entry 8ahm coloured by chain, top view.
Source organisms:
Primary publication:
Synthesis and Biological Evaluation of C(13)/C(13')-Bis(desmethyl)disorazole Z.
Bold CP, Lucena-Agell D, Oliva MÁ, Díaz JF, Altmann KH
OpenAccess logo Angew Chem Int Ed Engl (2022)
PMID: 36281761

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-274120 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Tubulin tyrosine ligase Chain: F

Ligands and Environments

9 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 5 x MG
Ligand GOL 1 x GOL
Ligand CA 4 x CA
Ligand DMS 2 x DMS
Ligand GDP 2 x GDP
Ligand MES 2 x MES
Ligand M5U 1 x M5U
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 105.047Å b: 157.8Å c: 181.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.241
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Synthesis and Biological Evaluation of C(13)/C(13')-Bis(desmethyl)disorazole Z.
Bold et al. (2023)

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