› 8alh

X-ray diffraction
1.86Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236511 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neuronal calcium sensor 1 Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 20.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62166 (Residues: 1-177; Coverage: 93%)
Gene names: FLUP, FREQ, NCS1
Sequence domains:
Synembryn-A Chain: P
Molecule details ›
Chain: P
Length: 30 amino acids
Theoretical weight: 3.09 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: Q80ZG1 (Residues: 400-429; Coverage: 6%)
Gene name: Ric8a

Ligands and Environments

6 bound ligands:
Ligand CA 2 x CA
Ligand NA 1 x NA
Ligand MG 1 x MG
Ligand 1PE 3 x 1PE
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P41212
Unit cell:
a: 56.861Å b: 56.861Å c: 134.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.231
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

1 mention without citation

The neuronal calcium sensor NCS-1 regulates the phosphorylation state and activity of the Gα chaperone and GEF Ric-8A.
Muñoz-Reyes et al. (2023)