› 8ao0

Solution NMR

Solution structure of nanoFAST/HBR-DOM2 complex

Released:
DOI: 10.2210/pdb8ao0/pdb
PDB entry 8ao0 coloured by chain, ensemble of 20 models, front view.
PDB entry 8ao0 coloured by chain, ensemble of 20 models, side view.
PDB entry 8ao0 coloured by chain, ensemble of 20 models, top view.
Source organism: synthetic construct
Primary publication:
Spatial Structure of NanoFAST in the Apo State and in Complex with its Fluorogen HBR-DOM2.
Lushpa VA, Baleeva NS, Goncharuk SA, Goncharuk MV, Arseniev AS, Baranov MS, Mineev KS
OpenAccess logo Int J Mol Sci 23 (2022)
PMID: 36232662

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.49 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 28-125; Coverage: 78%)
Gene name: pyp
Sequence domains: PAS fold

Ligands and Environments

1 bound ligand:
Ligand O1F 1 x O1F
1 modified residue:
Ligand GGL 1 x GGL

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Dynamic Structures of Bioactive Proteins as Determined by Nuclear Magnetic Resonance.
Toke et al. (2023)

1 mention without citation

Spatial Structure of NanoFAST in the Apo State and in Complex with its Fluorogen HBR-DOM2.
Lushpa et al. (2022)