› 8as2

X-ray diffraction
3.2Å resolution

Structure of arrestin2 in complex with 4P CCR5 phosphopeptide and Fab30

Released:
DOI: 10.2210/pdb8as2/pdb
PDB entry 8as2 coloured by chain, front view.
PDB entry 8as2 coloured by chain, side view.
PDB entry 8as2 coloured by chain, top view.
Source organisms:
Primary publication:
A key GPCR phosphorylation motif discovered in arrestin2⋅CCR5 phosphopeptide complexes.
Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK, Grzesiek S
Mol Cell (2023)
PMID: 37244255

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-273810 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-arrestin-1 Chain: A
Molecule details ›
Chain: A
Length: 359 amino acids
Theoretical weight: 40.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49407 (Residues: 1-359; Coverage: 86%)
Gene names: ARR1, ARRB1
Sequence domains:
C-C chemokine receptor type 5 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P51681 (Residues: 331-352; Coverage: 6%)
Gene names: CCR5, CMKBR5
Fab30 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 233 amino acids
Theoretical weight: 25.05 KDa
Source organism: Phage display vector pTDisp
Expression system: Escherichia coli
Fab30 light chain Chain: L
Molecule details ›
Chain: L
Length: 220 amino acids
Theoretical weight: 23.99 KDa
Source organism: Phage display vector pTDisp
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I212121
Unit cell:
a: 116.638Å b: 122.178Å c: 145.559Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.281 0.28 0.316
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided
  • Escherichia coli

Quick links

Citations

2 review citations

Structure, function and drug discovery of GPCR signaling.
Cheng et al. (2023)
1 more