› 8asg

Electron Microscopy
3.2Å resolution

Structure of the SFTSV L protein bound in a resting state [RESTING]

Released:
DOI: 10.2210/pdb8asg/pdb
PDB entry 8asg coloured by chain, front view.
PDB entry 8asg coloured by chain, side view.
PDB entry 8asg coloured by chain, top view.
Source organism: SFTS virus AH12
Primary publication:
Structural insights into viral genome replication by the severe fever with thrombocytopenia syndrome virus L protein.
Williams HM, Thorkelsson SR, Vogel D, Milewski M, Busch C, Cusack S, Grünewald K, Quemin ERJ, Rosenthal M
OpenAccess logo Nucleic Acids Res (2023)
PMID: 36651274
Related structures: EMD-15615

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197378 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2084 amino acids
Theoretical weight: 235.7 KDa
Source organism: SFTS virus AH12
Expression system: Trichoplusia ni
UniProt:
  • Canonical: U3GU88 (Residues: 1-2084; Coverage: 100%)
Sequence domains:
RNA (5'-R(*GP*AP*UP*CP*UP*GP*GP*GP*CP*GP*GP*UP*CP*UP*UP*UP*GP*UP*GP*U*)-3') Chains: D, T
Molecule details ›
Chains: D, T
Length: 20 nucleotides
Theoretical weight: 6.41 KDa
Source organism: SFTS virus AH12
Expression system: Not provided
RNA (5'-R(*AP*CP*AP*CP*AP*GP*AP*GP*AP*CP*GP*CP*CP*CP*AP*GP*AP*UP*GP*A*)-3') Chain: P
Molecule details ›
Chain: P
Length: 20 nucleotides
Theoretical weight: 6.45 KDa
Source organism: SFTS virus AH12
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-15615
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links

Citations

7 citation in other articles

Structural and functional characterization of the Sin Nombre virus L protein.
Meier et al. (2023)
6 more

2 mentions without citation

The mechanism of genome replication and transcription in bunyaviruses.
Malet et al. (2023)
1 more