› 8asi

Electron Microscopy
2.9Å resolution

Four subunit cytochrome b-c1 complex from Rhodobacter sphaeroides in native nanodiscs - consensus refinement in the b-b conformation

Released:
DOI: 10.2210/pdb8asi/pdb
PDB entry 8asi coloured by chain, front view.
PDB entry 8asi coloured by chain, side view.
PDB entry 8asi coloured by chain, top view.
Source organism: Cereibacter sphaeroides 2.4.1
Primary publication:
Cryo-EM structure of the four-subunit Rhodobacter sphaeroides cytochrome bc1 complex in styrene maleic acid nanodiscs.
Swainsbury DJK, Hawkings FR, Martin EC, Musiał S, Salisbury JH, Jackson PJ, Farmer DA, Johnson MP, Siebert CA, Hitchcock A, Hunter CN
Proc Natl Acad Sci U S A 120 e2217922120 (2023)
PMID: 36913593
Related structures: EMD-15616

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-174590 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: A, E
Molecule details ›
Chains: A, E
Length: 187 amino acids
Theoretical weight: 19.93 KDa
Source organism: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3IY09 (Residues: 1-187; Coverage: 100%)
Gene names: RSP_1396, fbcF
Sequence domains:
Cytochrome b Chains: B, F
Molecule details ›
Chains: B, F
Length: 445 amino acids
Theoretical weight: 50.09 KDa
Source organism: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3IY10 (Residues: 1-445; Coverage: 100%)
Gene names: RSP_1395, fbcB
Sequence domains:
Cytochrome c1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 285 amino acids
Theoretical weight: 30.66 KDa
Source organism: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3IY11 (Residues: 1-285; Coverage: 100%)
Gene names: RSP_1394, fbcC
Sequence domains: Cytochrome C1 family
Cytochrome b-c1 subunit IV Chains: D, H
Molecule details ›
Chains: D, H
Length: 124 amino acids
Theoretical weight: 14.42 KDa
Source organism: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3J2Z2 (Residues: 1-124; Coverage: 100%)
Gene names: RSP_2687, fbcQ

Ligands and Environments

5 bound ligands:
Ligand FES 2 x FES
Ligand PEE 12 x PEE
Ligand HEM 4 x HEM
Ligand HEC 2 x HEC
Ligand U10 1 x U10
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-15616

Quick links

Citations

1 review citation

Recent advances in membrane mimetics for membrane protein research.
Young JW. (2023)
2 other articles