› 8at2

Electron Microscopy
7.7Å resolution

Structure of the augmin TIII subcomplex

Released:
DOI: 10.2210/pdb8at2/pdb
PDB entry 8at2 coloured by chain, front view.
PDB entry 8at2 coloured by chain, side view.
PDB entry 8at2 coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
The augmin complex architecture reveals structural insights into microtubule branching.
Zupa E, Würtz M, Neuner A, Hoffmann T, Rettel M, Böhler A, Vermeulen BJA, Eustermann S, Schiebel E, Pfeffer S
OpenAccess logo Nat Commun 13 5635 (2022)
PMID: 36163468
Related structures: EMD-15631

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103603 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HAUS augmin-like complex subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 286 amino acids
Theoretical weight: 32.68 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: A0A8J1L9M8 (Residues: 1-286; Coverage: 100%)
Gene name: haus1.L
HAUS augmin-like complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 597 amino acids
Theoretical weight: 68.11 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q6DCY9 (Residues: 1-597; Coverage: 100%)
Gene name: haus3
Sequence domains: HAUS augmin-like complex subunit 3
HAUS augmin-like complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 353 amino acids
Theoretical weight: 41.26 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q4V7I1 (Residues: 1-353; Coverage: 100%)
Gene names: MGC115689, haus4, haus4.L
Sequence domains: HAUS augmin-like complex subunit 4
HAUS augmin-like complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 666 amino acids
Theoretical weight: 77.36 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: A0A1L8FPI2 (Residues: 1-666; Coverage: 100%)
Gene name: haus5.L
Sequence domains: HAUS augmin-like complex subunit 5

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.7Å
Relevant EMDB volumes: EMD-15631
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017

Quick links

Citations

5 citation in other articles

Augmin is a Ran-regulated spindle assembly factor.
Kraus et al. (2023)
4 more