› 8at4

Electron Microscopy
33Å resolution

Structure of the augmin holocomplex in closed conformation

Released:
DOI: 10.2210/pdb8at4/pdb
PDB entry 8at4 coloured by chain, front view.
PDB entry 8at4 coloured by chain, side view.
PDB entry 8at4 coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
The augmin complex architecture reveals structural insights into microtubule branching.
Zupa E, Würtz M, Neuner A, Hoffmann T, Rettel M, Böhler A, Vermeulen BJA, Eustermann S, Schiebel E, Pfeffer S
OpenAccess logo Nat Commun 13 5635 (2022)
PMID: 36163468
Related structures: EMD-15633

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-103602 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
HAUS augmin-like complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 32.68 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: A0A8J1L9M8 (Residues: 1-286; Coverage: 100%)
Gene name: haus1.L
HAUS augmin-like complex subunit 3 Chain: B
Molecule details ›
Chain: B
Length: 597 amino acids
Theoretical weight: 68.11 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q6DCY9 (Residues: 1-597; Coverage: 100%)
Gene name: haus3
Sequence domains: HAUS augmin-like complex subunit 3
HAUS augmin-like complex subunit 4 Chain: C
Molecule details ›
Chain: C
Length: 353 amino acids
Theoretical weight: 41.26 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q4V7I1 (Residues: 1-353; Coverage: 100%)
Gene names: MGC115689, haus4, haus4.L
Sequence domains: HAUS augmin-like complex subunit 4
HAUS augmin-like complex subunit 5 Chain: D
Molecule details ›
Chain: D
Length: 666 amino acids
Theoretical weight: 77.36 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: A0A1L8FPI2 (Residues: 1-666; Coverage: 100%)
Gene name: haus5.L
Sequence domains: HAUS augmin-like complex subunit 5
MGC82377 protein Chain: E
Molecule details ›
Chain: E
Length: 222 amino acids
Theoretical weight: 25.14 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q6INL9 (Residues: 1-222; Coverage: 100%)
Gene names: MGC82377, cep27, haus2, haus2.L
Sequence domains: HAUS augmin-like complex subunit 2
HAUS augmin-like complex subunit 6 N-terminal domain-containing protein Chain: F
Molecule details ›
Chain: F
Length: 978 amino acids
Theoretical weight: 110.86 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: A0JPI0 (Residues: 1-978; Coverage: 100%)
Gene names: LOC100036802, haus6, haus6.L
Sequence domains: HAUS augmin-like complex subunit 6 N-terminus
HAUS augmin-like complex subunit 7 Chain: G
Molecule details ›
Chain: G
Length: 348 amino acids
Theoretical weight: 39.38 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: B1H1T5 (Residues: 1-348; Coverage: 100%)
Gene names: LOC100158301, haus7, haus7.S, uchl5ip, uip1
HAUS augmin-like complex subunit 8 Chain: H
Molecule details ›
Chain: H
Length: 367 amino acids
Theoretical weight: 41.14 KDa
Source organism: Xenopus laevis
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q0IHJ3 (Residues: 1-367; Coverage: 100%)
Gene names: haus8, hice1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 33Å
Relevant EMDB volumes: EMD-15633
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017

Quick links

Citations

4 citation in other articles

Integrated model of the vertebrate augmin complex.
Travis et al. (2023)
3 more