› 8ate

X-ray diffraction
1.92Å resolution

Galacturonic acid oxidase from Citrus sinensis

Released:
DOI: 10.2210/pdb8ate/pdb
PDB entry 8ate coloured by chain, front view.
PDB entry 8ate coloured by chain, side view.
PDB entry 8ate coloured by chain, top view.
Source organism: Citrus sinensis
Primary publication:
Biochemical and Structural Characterization of a Uronic Acid Oxidase from Citrus sinensis
Boverio A, van Beek HL, Savino S, Ranoux A, Huijgen WJJ, Raaijmakers HWC, Fraaije MW, Loncar N
ChemCatChem (2023)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240562 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 509 amino acids
Theoretical weight: 57.32 KDa
Source organism: Citrus sinensis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: V4TNJ4 (Residues: 30-537; Coverage: 100%)
Gene name: CICLE_v10014838mg
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand FAD 1 x FAD
Ligand NAG 1 x NAG
Ligand CL 1 x CL
Ligand IOD 2 x IOD
Ligand NA 2 x NA
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P3221
Unit cell:
a: 101.653Å b: 101.653Å c: 108.746Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.244
Expression system: Komagataella pastoris

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