› 8av2

X-ray diffraction
1.75Å resolution

Crystal structure for the FnIII module of mouse LEP-R in complex with the anti-LEP-R nanobody VHH-4.80

Released:
DOI: 10.2210/pdb8av2/pdb
PDB entry 8av2 coloured by chain, front view.
PDB entry 8av2 coloured by chain, side view.
PDB entry 8av2 coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of receptor assembly via the pleiotropic adipokine Leptin.
Tsirigotaki A, Dansercoer A, Verschueren KHG, Marković I, Pollmann C, Hafer M, Felix J, Birck C, Van Putte W, Catteeuw D, Tavernier J, Fernando Bazan J, Piehler J, Savvides SN, Verstraete K
Nat Struct Mol Biol (2023)
PMID: 36959263

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273800 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Leptin receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.78 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P48356 (Residues: 633-827; Coverage: 17%)
Gene names: Db, Lepr, Obr
anti-mLEPR VHH 4-80 Chains: C, D
Molecule details ›
Chains: C, D
Length: 141 amino acids
Theoretical weight: 15.58 KDa
Source organism: Lama glama
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand NAG 4 x NAG
Ligand SO4 10 x SO4
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 53.192Å b: 85.876Å c: 144.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.233
Expression system: Homo sapiens

Quick links

Citations

4 citation in other articles

Structural insights into the mechanism of leptin receptor activation.
Saxton et al. (2023)
3 more