› 8avo

Electron Microscopy
6.84Å resolution

Human leptin in complex with the human LEP-R ectodomain fused to a C-terminal trimeric isoleucine GCN4 zipper (open 3:3 model).

Released:
DOI: 10.2210/pdb8avo/pdb
PDB entry 8avo coloured by chain, front view.
PDB entry 8avo coloured by chain, side view.
PDB entry 8avo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of receptor assembly via the pleiotropic adipokine Leptin.
Tsirigotaki A, Dansercoer A, Verschueren KHG, Marković I, Pollmann C, Hafer M, Felix J, Birck C, Van Putte W, Catteeuw D, Tavernier J, Fernando Bazan J, Piehler J, Savvides SN, Verstraete K
Nat Struct Mol Biol (2023)
PMID: 36959263
Related structures: EMD-15683

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214909 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Leptin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 171 amino acids
Theoretical weight: 18.61 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P41159 (Residues: 22-167; Coverage: 100%)
Gene names: LEP, OB, OBS
Sequence domains: Leptin
Leptin receptor Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 868 amino acids
Theoretical weight: 98.82 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P48357 (Residues: 22-839; Coverage: 72%)
  • Best match: P48357-5 (Residues: 22-839)
Gene names: DB, LEPR, OBR
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.84Å
Relevant EMDB volumes: EMD-15683
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Structural insights into the mechanism of leptin receptor activation.
Saxton et al. (2023)