› 8ax3

X-ray diffraction
1.59Å resolution

Structure of recombinant human beta-glucocerebrosidase in complex with L-carbaxylosyl fluoride

Released:
DOI: 10.2210/pdb8ax3/pdb
PDB entry 8ax3 coloured by chain, front view.
PDB entry 8ax3 coloured by chain, side view.
PDB entry 8ax3 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Rowland RJ, Davies GJ

Function and Biology Details

Reactions catalysed: 
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Lysosomal acid glucosylceramidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 55.66 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GBA1, GC, GLUC
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
7 bound ligands:
Ligand SO4 6 x SO4
Ligand EDO 47 x EDO
Ligand NAG 2 x NAG
Ligand OD0 4 x OD0
Ligand NA 1 x NA
Ligand GOL 1 x GOL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 52.92Å b: 155.984Å c: 68.036Å
α: 90° β: 101.964° γ: 90°
R-values:
R R work R free
0.183 0.182 0.212
Expression system: Trichoplusia ni

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