› 8b0h

Electron Microscopy
3.3Å resolution

2C9, C5b9-CD59 cryoEM structure

Released:
DOI: 10.2210/pdb8b0h/pdb
PDB entry 8b0h coloured by chain, front view.
PDB entry 8b0h coloured by chain, side view.
PDB entry 8b0h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for membrane attack complex inhibition by CD59.
Couves EC, Gardner S, Voisin TB, Bickel JK, Stansfeld PJ, Tate EW, Bubeck D
OpenAccess logo Nat Commun 14 890 (2023)
PMID: 36797260
Related structures: EMD-15781

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133865 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
CD59 glycoprotein Chain: G
Molecule details ›
Chain: G
Length: 128 amino acids
Theoretical weight: 14.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13987 (Residues: 1-128; Coverage: 100%)
Gene names: CD59, MIC11, MIN1, MIN2, MIN3, MSK21
Sequence domains: u-PAR/Ly-6 domain
Complement component C8 beta chain Chain: D
Molecule details ›
Chain: D
Length: 591 amino acids
Theoretical weight: 67.14 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07358 (Residues: 1-591; Coverage: 100%)
Gene name: C8B
Sequence domains:
Complement component C8 gamma chain Chain: F
Complement component C8 alpha chain Chain: E
Complement C5 Chain: A
Complement component C7 Chain: C
Complement component C6 Chain: B
Complement component C9 Chains: H, I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-15781
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

A guide to complement biology, pathology and therapeutic opportunity.
Mastellos et al. (2024)
1 more