› 8b0i

Electron Microscopy
4.28Å resolution

CryoEM structure of bacterial RapZ.GlmZ complex central to the control of cell envelope biogenesis

Released:
DOI: 10.2210/pdb8b0i/pdb
PDB entry 8b0i coloured by chain, front view.
PDB entry 8b0i coloured by chain, side view.
PDB entry 8b0i coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.
Islam MS, Hardwick SW, Quell L, Durica-Mitic S, Chirgadze DY, Görke B, Luisi BF
OpenAccess logo EMBO J e112574 (2022)
PMID: 36504162
Related structures: EMD-15784

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141687 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNase adapter protein RapZ Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 284 amino acids
Theoretical weight: 32.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A894 (Residues: 1-284; Coverage: 100%)
Gene names: JW3172, b3205, rapZ, yhbJ
Sequence domains:
GlmZ small regulatory RNA Chain: K
Molecule details ›
Chain: K
Length: 207 nucleotides
Theoretical weight: 66.06 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
Sequence domains: GlmZ RNA activator of glmS mRNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.28Å
Relevant EMDB volumes: EMD-15784
Expression system: Escherichia coli

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Citations

1 review citation

Recent Advances in Peptidoglycan Synthesis and Regulation in Bacteria.
Galinier et al. (2023)
3 other articles