› 8b20

X-ray diffraction
1.78Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 25.63 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F6IAY7 (Residues: 29-270; Coverage: 90%)
Gene names: blaNDM-1, blaNDM1
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand CA 3 x CA
Ligand EDO 3 x EDO
Ligand OQK 1 x OQK
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 77.068Å b: 70.444Å c: 73.803Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.207
Expression system: Escherichia coli BL21(DE3)

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