› 8b3b

X-ray diffraction
1.95Å resolution

Structure of metacyclic VSG (mVSG) 531 from Trypanosoma brucei

Released:
DOI: 10.2210/pdb8b3b/pdb
PDB entry 8b3b coloured by chain, front view.
PDB entry 8b3b coloured by chain, side view.
PDB entry 8b3b coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Structural similarities between the metacyclic and bloodstream form variant surface glycoproteins of the African trypanosome.
Chandra M, Đaković S, Foti K, Zeelen JP, van Straaten M, Aresta-Branco F, Tihon E, Lübbehusen N, Ruppert T, Glover L, Papavasiliou FN, Stebbins CE
PLoS Negl Trop Dis 17 e0011093 (2023)
PMID: 36780870

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-126027 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Trypanosome variant surface glycoprotein A-type N-terminal domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 371 amino acids
Theoretical weight: 38.78 KDa
Source organism: Trypanosoma brucei
UniProt:
  • Canonical: M4SYA9 (Residues: 29-399; Coverage: 80%)
Sequence domains: Trypanosome variant surface glycoprotein (A-type)
Trypanosome variant surface glycoprotein A-type N-terminal domain-containing protein Chains: D, G, H
Molecule details ›
Chains: D, G, H
Length: 372 amino acids
Theoretical weight: 38.87 KDa
Source organism: Trypanosoma brucei
UniProt:
  • Canonical: M4SYA9 (Residues: 29-400; Coverage: 80%)
Sequence domains: Trypanosome variant surface glycoprotein (A-type)
Trypanosome variant surface glycoprotein A-type N-terminal domain-containing protein Chains: B, E, F
Molecule details ›
Chains: B, E, F
Length: 370 amino acids
Theoretical weight: 38.7 KDa
Source organism: Trypanosoma brucei
UniProt:
  • Canonical: M4SYA9 (Residues: 29-398; Coverage: 80%)
Sequence domains: Trypanosome variant surface glycoprotein (A-type)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 12 x NAG
Ligand BMA 5 x BMA
Ligand MAN 19 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 50.107Å b: 170.885Å c: 163.652Å
α: 90° β: 90.71° γ: 90°
R-values:
R R work R free
0.202 0.2 0.238

Quick links

Citations

2 citation in other articles

A structural classification of the variant surface glycoproteins of the African trypanosome.
Đaković et al. (2023)
1 more

1 mention without citation

Step by step evolution of Indeterminate Domain (IDD) transcriptional regulators: from algae to angiosperms.
Prochetto et al. (2020)