8b5r

Electron Microscopy
6.1Å resolution

p97-p37-SPI substrate complex

Released:
Source organism: Homo sapiens
Related structures: EMD-15861

Function and Biology Details

Reactions catalysed:
ATP + H(2)O = ADP + phosphate
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-217303 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Transitional endoplasmic reticulum ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 812 amino acids
Theoretical weight: 90.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P55072 (Residues: 2-806; Coverage: 100%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
I3 sequence being threaded through the p97 channel Chain: I
Molecule details ›
Chain: I
Length: 22 amino acids
Theoretical weight: 1.89 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit Chain: P
Molecule details ›
Chain: P
Length: 323 amino acids
Theoretical weight: 37.03 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P36873 (Residues: 1-323; Coverage: 100%)
Gene name: PPP1CC
Sequence domains:
Protein phosphatase 1 regulatory subunit 7 Chain: S
Molecule details ›
Chain: S
Length: 360 amino acids
Theoretical weight: 41.62 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15435 (Residues: 1-360; Coverage: 100%)
Gene names: PPP1R7, SDS22
Sequence domains:
UBX domain-containing protein 2B Chain: X
Molecule details ›
Chain: X
Length: 11 amino acids
Theoretical weight: 1.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q14CS0 (Residues: 215-225; Coverage: 3%)
Gene name: UBXN2B
UBX domain-containing protein 2B Chain: Y
Molecule details ›
Chain: Y
Length: 86 amino acids
Theoretical weight: 9.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q14CS0 (Residues: 246-331; Coverage: 26%)
Gene name: UBXN2B
Sequence domains: UBX domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.1Å
Relevant EMDB volumes: EMD-15861
Expression systems:
  • Trichoplusia ni
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'