› 8b6g

Electron Microscopy
3Å resolution

Cryo-EM structure of succinate dehydrogenase complex (complex-II) in respiratory supercomplex of Tetrahymena thermophila

Released:
DOI: 10.2210/pdb8b6g/pdb
PDB entry 8b6g coloured by chain, front view.
PDB entry 8b6g coloured by chain, side view.
PDB entry 8b6g coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Structural basis of mitochondrial membrane bending by the I-II-III2-IV2 supercomplex.
Mühleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Maréchal A, Sharma V, Amunts A
OpenAccess logo Nature 615 934-938 (2023)
PMID: 36949187
Related structures: EMD-15866

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-271320 (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
Diphthamide synthesis protein Chain: CH
Molecule details ›
Chain: CH
Length: 195 amino acids
Theoretical weight: 22.89 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7LX66 (Residues: 1-195; Coverage: 100%)
Gene name: TTHERM_00658950
Transmembrane protein, putative Chain: CM
Molecule details ›
Chain: CM
Length: 76 amino acids
Theoretical weight: 9.17 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22HD6 (Residues: 1-76; Coverage: 100%)
Gene name: TTHERM_00637670
Transposase Chain: CL
Molecule details ›
Chain: CL
Length: 89 amino acids
Theoretical weight: 11.03 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: W7XBF5 (Residues: 1-89; Coverage: 100%)
Gene name: TTHERM_000287919
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial Chain: CA
Molecule details ›
Chain: CA
Length: 636 amino acids
Theoretical weight: 70.08 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23DI3 (Residues: 1-636; Coverage: 100%)
Gene name: TTHERM_00047080
Sequence domains:
DUF4885 domain-containing protein Chain: CI
Molecule details ›
Chain: CI
Length: 114 amino acids
Theoretical weight: 12.98 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q22YL0 (Residues: 1-114; Coverage: 100%)
Gene name: TTHERM_00601860
succinate dehydrogenase Chain: CB
Molecule details ›
Chain: CB
Length: 312 amino acids
Theoretical weight: 36 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7M403 (Residues: 1-312; Coverage: 100%)
Gene name: TTHERM_00241700
Sequence domains:
Transmembrane protein, putative Chain: CF
Molecule details ›
Chain: CF
Length: 296 amino acids
Theoretical weight: 35.12 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q248F8 (Residues: 1-296; Coverage: 100%)
Gene name: TTHERM_00532090
SDHTT3 Chain: CG
Molecule details ›
Chain: CG
Length: 198 amino acids
Theoretical weight: 23.63 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: I7MEX7 (Residues: 1-198; Coverage: 100%)
Gene name: TTHERM_00283850
Transmembrane protein, putative Chain: CK
Molecule details ›
Chain: CK
Length: 93 amino acids
Theoretical weight: 11.19 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q24CW6 (Residues: 1-93; Coverage: 100%)
Gene name: TTHERM_00713350
NmrA-like domain-containing protein Chain: CE
Molecule details ›
Chain: CE
Length: 322 amino acids
Theoretical weight: 36.38 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q24I09 (Residues: 1-322; Coverage: 100%)
Gene name: TTHERM_00571650
Transmembrane protein, putative Chain: CJ
Molecule details ›
Chain: CJ
Length: 103 amino acids
Theoretical weight: 11.96 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23S01 (Residues: 1-103; Coverage: 100%)
Gene name: TTHERM_00626980
Transmembrane protein, putative Chain: CN
Molecule details ›
Chain: CN
Length: 62 amino acids
Theoretical weight: 7.24 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: W7XF00 (Residues: 1-62; Coverage: 100%)
Gene name: TTHERM_001034353
Transmembrane protein Chain: CC
Molecule details ›
Chain: CC
Length: 60 amino acids
Theoretical weight: 7.04 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q23RH8 (Residues: 1-60; Coverage: 100%)
Gene name: TTHERM_00387120
SDHTT11 Chain: CO
Molecule details ›
Chain: CO
Length: 43 amino acids
Theoretical weight: 5.16 KDa
Source organism: Tetrahymena thermophila
SDHD Chain: CD
Molecule details ›
Chain: CD
Length: 44 amino acids
Theoretical weight: 5.57 KDa
Source organism: Tetrahymena thermophila

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand HEC 1 x HEC
8 bound ligands:
Ligand CDL 7 x CDL
Ligand CA 2 x CA
Ligand FES 1 x FES
Ligand SF4 1 x SF4
Ligand F3S 1 x F3S
Ligand 3PE 3 x 3PE
Ligand PC1 1 x PC1
Ligand UQ8 1 x UQ8
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-15866

Quick links

Citations

7 review citations

The mystery of massive mitochondrial complexes: the apicomplexan respiratory chain.
Maclean et al. (2022)
6 more