› 8b6h

Electron Microscopy
2.6Å resolution

Cryo-EM structure of cytochrome c oxidase dimer (complex IV) from respiratory supercomplex of Tetrahymena thermophila

Released:
DOI: 10.2210/pdb8b6h/pdb
PDB entry 8b6h coloured by chain, front view.
PDB entry 8b6h coloured by chain, side view.
PDB entry 8b6h coloured by chain, top view.
Source organism: Tetrahymena thermophila SB210
Primary publication:
Structural basis of mitochondrial membrane bending by the I-II-III2-IV2 supercomplex.
Mühleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Maréchal A, Sharma V, Amunts A
OpenAccess logo Nature 615 934-938 (2023)
PMID: 36949187
Related structures: EMD-15867

Function and Biology Details

Reaction catalysed: 
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 106-mer (preferred)
PDBe Complex ID:
PDB-CPX-269844 (preferred)
Entry contents:
53 distinct polypeptide molecules
Macromolecules (53 distinct):
Cytochrome c oxidase subunit 1 Chains: DA, Da
Molecule details ›
Chains: DA, Da
Length: 688 amino acids
Theoretical weight: 80.58 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q950Y4 (Residues: 1-688; Coverage: 100%)
Gene name: cox1
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome c oxidase polypeptide II Chains: DB, Db
Molecule details ›
Chains: DB, Db
Length: 604 amino acids
Theoretical weight: 72.39 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q950Y9 (Residues: 1-604; Coverage: 100%)
Gene name: cox2
Sequence domains: Cytochrome C oxidase subunit II, periplasmic domain
Ymf68 Chains: DC, Dc
Cytochrome C oxidase subunit Vb protein Chains: DD, Dd
Transmembrane protein, putative Chains: DE, De
Structural protein Chains: DF, Df
Transmembrane protein, putative Chains: DG, Dg
Transmembrane protein, putative Chains: DH, Dh
Transmembrane protein Chains: DI, Di
Transmembrane protein, putative Chains: DJ, Dj
CTF/NF-I domain-containing protein Chains: DK, Dk
Transmembrane protein, putative Chains: DM, Dm
Ymf67 Chains: DN, Dn
Protein phosphatase 2C, putative Chains: DO, Do
SURF1-like protein Chains: DP, Dp
TraB family protein Chains: DQ, Dq
Transmembrane protein, putative Chains: DR, Dr
Oxoglutarate/malate translocator protein, putative Chains: DS, Ds
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial Chains: DT, Dt
Carrier protein Chains: DU, Du
2-oxoglutarate/malate carrier protein Chains: DV, Dv
SURF1-like protein Chains: DW, Dw
Transmembrane protein Chains: DX, Dx
Transmembrane protein Chains: DY, Dy
39S ribosomal protein L9, mitochondrial Chains: DZ, Dz
Transmembrane protein Chains: EA, Ea
Transmembrane protein, putative Chains: EB, Eb
COXTT27 Chains: EC, Ec
Ymf75 Chains: ED, Ed
Mobilization protein Chains: EE, Ee
Iron-binding zinc finger CDGSH type domain-containing protein Chains: EF, Ef
COXTT28 Chains: EG, Eg
Transmembrane protein, putative Chains: EH, Eh
Transmembrane protein Chains: EI, Ei
Decapping nuclease Chains: EV, Ev
Complex III subunit VII Chains: EK, Ek
Transmembrane protein, putative Chains: EL, El
Transmembrane protein, putative Chains: EM, Em
Transmembrane protein Chains: EN, En
Transmembrane protein, putative Chains: EO, Eo
Phage protein Chains: EP, Ep
Transmembrane protein, putative Chains: EQ, Eq
Lysozyme Chains: ER, Er
Ymf70 Chains: ES, Es
Tim10/DDP family zinc finger protein Chains: ET, Et
Transmembrane protein Chains: EU, Eu
YflT domain-containing protein Chains: EJ, Ej
Cullin domain-containing protein Chains: EW, Ew
Tim10/DDP family zinc finger protein Chains: EX, Ex
Annexin Chains: EY, Ey
Transposase Chains: EZ, Ez
Mitochondrial import inner membrane translocase subunit Chains: FA, Fa
Chromosome condensation regulator RCC1 repeat protein Chains: DL, Dl

Ligands and Environments

15 bound ligands:
Ligand HEA 4 x HEA
Ligand CU 2 x CU
Ligand MG 2 x MG
Ligand CDL 118 x CDL
Ligand PC1 54 x PC1
Ligand 3PE 34 x 3PE
Ligand LPP 8 x LPP
Ligand CA 2 x CA
Ligand CUA 2 x CUA
Ligand ZN 2 x ZN
Ligand K 1 x K
Ligand UQ8 8 x UQ8
Ligand HEM 2 x HEM
Ligand FES 4 x FES
Ligand ATP 2 x ATP
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 4 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.6Å
Relevant EMDB volumes: EMD-15867

Quick links

Citations

7 review citations

The mystery of massive mitochondrial complexes: the apicomplexan respiratory chain.
Maclean et al. (2022)
6 more