› 8b6j

Electron Microscopy
2.8Å resolution

Cryo-EM structure of cytochrome bc1 complex (complex-III) from respiratory supercomplex of Tetrahymena thermophila

Released:
DOI: 10.2210/pdb8b6j/pdb
PDB entry 8b6j coloured by chain, front view.
PDB entry 8b6j coloured by chain, side view.
PDB entry 8b6j coloured by chain, top view.
Source organism: Tetrahymena thermophila SB210
Primary publication:
Structural basis of mitochondrial membrane bending by the I-II-III2-IV2 supercomplex.
Mühleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Maréchal A, Sharma V, Amunts A
OpenAccess logo Nature 615 934-938 (2023)
PMID: 36949187
Related structures: EMD-15868

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
PDBe Complex ID:
PDB-CPX-125279 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
Peptidase M16 inactive domain protein Chains: A, a
Molecule details ›
Chains: A, a
Length: 513 amino acids
Theoretical weight: 58.02 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7MGU2 (Residues: 1-513; Coverage: 100%)
Gene name: TTHERM_00502380
Sequence domains:
Peptidase M16 C-terminal domain-containing protein Chains: B, b
Molecule details ›
Chains: B, b
Length: 482 amino acids
Theoretical weight: 53.03 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7MJ25 (Residues: 1-482; Coverage: 100%)
Gene name: TTHERM_00836690
Sequence domains: Peptidase M16 inactive domain
Cytochrome b/b6 N-terminal region profile domain-containing protein Chains: C, c
Molecule details ›
Chains: C, c
Length: 426 amino acids
Theoretical weight: 50.64 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q950Z1 (Residues: 1-426; Coverage: 100%)
Gene name: cob
Sequence domains: Cytochrome b/b6/petB
Cytochrome protein c1 Chains: D, d
Molecule details ›
Chains: D, d
Length: 319 amino acids
Theoretical weight: 37.62 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q24IM5 (Residues: 1-319; Coverage: 100%)
Gene name: TTHERM_00918500
Sequence domains: Cytochrome C1 family
Rieske domain-containing protein Chains: E, e
Molecule details ›
Chains: E, e
Length: 269 amino acids
Theoretical weight: 30.7 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7MIC7 (Residues: 1-269; Coverage: 100%)
Gene name: TTHERM_00295080
Sequence domains: Rieske [2Fe-2S] domain
Ubiquinol-cytochrome C reductase hinge domain-containing protein Chains: F, f
Molecule details ›
Chains: F, f
Length: 86 amino acids
Theoretical weight: 9.89 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q23K66 (Residues: 1-86; Coverage: 100%)
Gene name: TTHERM_00194690
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
UQCRTT1 Chains: G, g
Molecule details ›
Chains: G, g
Length: 328 amino acids
Theoretical weight: 39.19 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: Q23F81 (Residues: 1-328; Coverage: 100%)
Gene name: TTHERM_00382330
Transmembrane protein, putative Chains: H, h
Molecule details ›
Chains: H, h
Length: 130 amino acids
Theoretical weight: 15.68 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7M484 (Residues: 1-130; Coverage: 100%)
Gene name: TTHERM_00765330
Transmembrane protein, putative Chains: I, i
Molecule details ›
Chains: I, i
Length: 119 amino acids
Theoretical weight: 14.26 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7MM45 (Residues: 1-119; Coverage: 100%)
Gene name: TTHERM_00456790
UQCRTT3/UP1 Chains: J, j
Molecule details ›
Chains: J, j
Length: 66 amino acids
Theoretical weight: 5.63 KDa
Source organism: Tetrahymena thermophila SB210
Transmembrane protein, putative Chains: K, k
Molecule details ›
Chains: K, k
Length: 62 amino acids
Theoretical weight: 7.46 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:
  • Canonical: I7MFL6 (Residues: 1-62; Coverage: 100%)
Gene name: TTHERM_00218930
UQCRTT2 Chains: L, l
Molecule details ›
Chains: L, l
Length: 41 amino acids
Theoretical weight: 4.85 KDa
Source organism: Tetrahymena thermophila SB210

Ligands and Environments


Cofactor: Ligand HEC 2 x HEC

Cofactor: Ligand HEM 4 x HEM
5 bound ligands:
Ligand PC1 15 x PC1
Ligand CDL 15 x CDL
Ligand UQ8 3 x UQ8
Ligand PEE 2 x PEE
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-15868

Quick links

Citations

7 review citations

The mystery of massive mitochondrial complexes: the apicomplexan respiratory chain.
Maclean et al. (2022)
6 more